Keith Robison has written another fantastic post on his Omics! Omics! blog which is a great read for two reasons.
First he looks at the issues regarding chromosome-size scaffolds that can now be produced with Hi-C sequencing approches. He then goes on to provide a brilliant overview of what the latest sequencing and mapping technologies mean for the field of genome assembly:
For high quality de novo genomes, the technology options appear to be converging for the moment on five basic technologies which can be mixed-and-matched.
- Hi-C (in vitro or in vivo)
- Rapid Physical Maps (BioNano Genomics)
- Linked Reads (10X, iGenomX)
- Oxford Nanopore
- Pacific Biosciences
- vanilla Illumina paired end
This second section should be required reading for anyone interested in genome assembly, particularly if you've been away for the field for a while.
Read the post: Chromosome-Scale Scaffolds And The State of Genome Assembly